ABSTRACT
In order to research the mechanism of guiding action of borneol in Suxiaojiuxin pills, the model of in vitro intestinal absorption, in vivo drug metabolism of mice and cell in vitro absorption model of Caco-2 were established firstly. All animal experiments were in accordance with the regulations of the Animal Ethics Committee of Nankai University. The results showed that the cumulative absorption quantity and absorption permeability of ferulic acid and ligustilide in the intestinal juice of Suxiaojiuxin pills group were significantly increased comparing with fake Suxiaojiuxin pills group, which don't contain borneol. By using borneol, the content of ferulic acid and ligustilide in the blood and tissues, such as heart, were added. The transepithelial resistance value and the content of horseradish peroxidase (HRP) in Caco-2 were rapidly decreased and increased, respectively. Due to further explore mechanism of promoting intestinal absorption of borneol for drugs, in this study, photosensitive probes of borneol were synthesized to capture its targets, and dual luciferase reporter system was used to evaluate its activity of calcium. It was found that it could make calcium overload by regulating transient receptor potential cation channel, subfamily M, member 8 (TrpM8). Then, the results of mass spectrometry imaging showed that the accumulation of ferulic acid in the heart was significantly increased by borneol, and the relaxation rate of rat thoracic aorta was enhanced obviously. In summary, the borneol in Suxiaojiuxin pills can expand cell space and increase intestinal permeability by acting on TrpM8, thus promoting the intestinal absorption, tissue distribution and target organ enrichment of drugs.
ABSTRACT
Spleen and Stomach is the central embodiment of Li Dongyuan's academic thought, which has an extensive and profound influence on the later physicians and clinical application. In this paper,based on the specific Herbal Prescription of Li Dong yuan’s Treatise on Spleen and Stomach,the use of the concept of wholism in TCM,as wel as the Thinking Characteristics of Syndrome Differentiation Dependent Therapy ,discussing the drug administer-ing characteristics of Spleen and Stomach in four aspects:the use of wind drugs,combined ascend and descend,adjusting the medication according to the climatic change of the four seasons,emphasizing dietetic therapy ,medicine and food have the same effect.Obtaining the fol owing conclusions :The Dong Yuan treatment of disease is good at starting from the spleen and stomach. Attach importance to the function of the spleen and stomach of qi activity,its legislation is rigorous, and the thought of harmony between man and nature,ect.The research is helpful to further study of Dong yuan ’s academic thought ,to provide a theoretical basis for treating spleen and stomach disease nowadays.